Brian Pena received his undergraduate degree from Louisiana State University where he graduated with a bachelor's degree in kinesiology. He went on to receive his Associate of Science in Nursing degree from Our Lady of the Lake College in Baton Rouge, and his Bachelor's of Science in Nursing from the University of Louisiana - Lafayette.

Brian attended Louisiana State University Health Sciences Center in New Orleans where he completed his Doctor of Nursing Practice degree in 2019. He is a member of the American Association of Nurse Anesthetists.